http://www.brownlab.ca/home daily 1.0 2023-02-11 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1471286678553-3EFHCJ5HNC5IO2SAAORQ/IMG_3464.jpg Home http://www.brownlab.ca/publications-1 daily 0.75 2026-07-20 http://www.brownlab.ca/publications-1/2026/7/20/inhibitors-of-nutrient-pathways-natures-overlooked-antibiotic-arsenal monthly 0.5 2026-08-07 http://www.brownlab.ca/publications-1/2026/7/20/a-streptomyces-megacluster-encodes-synergistic-biotin-targeting-antibiotics monthly 0.5 2026-07-20 http://www.brownlab.ca/publications-1/2026/7/20/synthemol-rl-a-flexible-reinforcement-learning-framework-for-designing-easily-synthesizable-antibiotics monthly 0.5 2026-07-20 http://www.brownlab.ca/publications-1/2026/4/3/butyrolactol-a-enhances-caspofungin-efficacy-via-flippase-inhibition-in-drug-resistant-fungi monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/evaluation-of-azithromycin-bicarbonate-against-multidrug-resistant-pathogens-in-topical-murine-models-of-infection monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/discovery-and-artificial-intelligence-guided-mechanistic-elucidation-of-a-narrow-spectrum-antibiotic monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/coniontins-lipopetaibiotics-active-against-candida-auris-identified-from-a-microbial-natural-product-fractionation-library monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/identifying-modulators-of-the-post-antibiotic-effect monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/butyrolactol-a-potentiates-caspofungin-efficacy-against-resistant-fungi-via-phospholipid-flippase-inhibition monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/synthemol-rl-a-flexible-reinforcement-learning-framework-for-designing-novel-and-synthesizable-antibiotics monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/cryo-em-analyses-unveil-details-of-mechanism-and-targocil-ii-mediated-inhibition-of-s-aureus-wta-transporter-targh monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/important-challenges-to-finding-new-leads-for-new-antibiotics monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/exploiting-the-fitness-cost-of-metallo-lactamase-expression-can-overcome-antibiotic-resistance-in-bacterial-pathogens monthly 0.5 2026-08-11 http://www.brownlab.ca/publications-1/2026/4/3/high-throughput-discovery-of-synthetic-siderophores-for-trojan-horse-antibiotics monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2025/11/17/a-screen-for-cell-envelope-stress-uncovers-an-inhibitor-of-prolipoprotein-diacylglyceryl-transferase-lgt-in-escherichia-coli monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/micro-plaque-assays-a-high-throughput-method-to-detect-isolate-and-characterize-bacteriophages monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/the-inactivation-of-tolc-sensitizes-escherichia-coli-to-perturbations-in-lipopolysaccharide-transport monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/a-platform-for-predicting-mechanism-of-action-based-on-bacterial-transcriptional-responses-identifies-an-unusual-dna-gyrase-inhibitor monthly 0.5 2026-08-07 http://www.brownlab.ca/publications-1/2026/4/3/cryo-em-analysis-of-s-aureus-tarl-a-polymerase-in-wall-teichoic-acid-biogenesis-central-to-virulence-and-antibiotic-resistance monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/a-mobile-crispri-collection-enables-genetic-interaction-studies-for-the-essential-genes-of-escherichia-coli monthly 0.5 2026-04-22 http://www.brownlab.ca/publications-1/2026/4/3/a-standardized-nomenclature-for-resistance-modifying-agents-in-the-comprehensive-antibiotic-resistance-database monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/central-metabolism-is-a-key-player-in-e-coli-biofilm-stimulation-by-sub-mic-antibiotics monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2026/4/3/inhibiting-fatty-acid-synthesis-overcomes-colistin-resistance monthly 0.5 2026-08-11 http://www.brownlab.ca/publications-1/2026/4/3/food-for-thought-opportunities-to-target-carbon-metabolism-in-antibacterial-drug-discovery monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/2025/11/17/a-bifunctional-spray-coating-reduces-contamination-on-surfaces-by-repelling-and-killing-pathogens monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr-c274j-amzj4-g42mg-y5xnt monthly 0.5 2026-04-22 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/9f289f3d-fc65-47bd-acf1-1ef29c4e0ac5/Picture1.png Publications - Nutrient stress is a target for new antibiotics - Make it stand out Whatever it is, the way you tell your story online can make all the difference. http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr-c274j-amzj4-g42mg monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr-c274j-amzj4 monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr-c274j monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr-c274j-amzj4-g42mg-sydn5-f8k9x monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7 monthly 0.5 2026-04-03 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/mmpxe monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/f35b5f95-6fa4-40db-90f1-6a0ab00728b0/1-s2.0-S2451945621003111-fx1_lrg.jpg Publications - Armeniaspirols inhibit the AAA+ proteases ClpXP and ClpYQ leading to cell division arrest in Gram-positive bacteria http://www.brownlab.ca/publications-1/mmpxe-2021 monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/chem-screen-vancomycin monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1623183315736-JM59VHPKX1H3LSUXQR5K/cb1c00179_0008.gif Publications - Chemical Screen for Vancomycin Antagonism Uncovers Probes of the Gram-Negative Outer Membrane http://www.brownlab.ca/publications-1/jsb monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/3b16cd0d-6de1-463b-8c25-9b1fff87b109/1-s2.0-S1047847721000381-ga1_lrg.jpg Publications - Crystallographic analysis of TarI and TarJ, a cytidylyltransferase and reductase pair for CDP-ribitol synthesis in Staphylococcus aureus wall teichoic acid biogenesis http://www.brownlab.ca/publications-1/systems-chem-bio monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1623181621621-D7SSBD7XGOAK08U8EYRD/ar1c00011_0005.gif Publications - Systems-Level Chemical Biology to Accelerate Antibiotic Drug Discovery http://www.brownlab.ca/publications-1/carbon-environments-r9akc monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1623180112752-2JOIFRGAIDRU9K5TOZ8J/aac.01925-20-f0001.jpg Publications - Physicochemical and Structural Parameters Contributing to the Antibacterial Activity and Efflux Susceptibility of Small-Molecule Inhibitors of Escherichia coli http://www.brownlab.ca/publications-1/phage-antibiotic-combo monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/carbon-environments monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1601855337787-KLNUNG3EI5QMX9LT6Y3Q/Screen+Shot+2020-10-04+at+7.48.36+PM.png Publications - Gene Dispensability in Escherichia coli Grown in Thirty Different Carbon Environments http://www.brownlab.ca/publications-1/om-disruption-resistance monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1612471302050-4PKJEJ6X3CVUWD1OQUIE/Screen+Shot+2021-02-04+at+3.39.37+PM.png Publications - Outer Membrane Disruption Overcomes Intrinsic, Acquired, and Spontaneous Antibiotic Resistance http://www.brownlab.ca/publications-1/macrolide-bicarbonate monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1601850482590-3KS4WKSEXRMVEW8M0B5Q/.png Publications - Overcoming Acquired and Native Macrolide Resistance with Bicarbonate http://www.brownlab.ca/publications-1/serumscreen monthly 0.5 2026-04-03 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1596205515104-D4YSC2FIA1GEEXSXOAWD/Brent_graph_abstract.jpg Publications - Genetic and Chemical Screening in Human Blood Serum Reveals Unique Antibacterial Targets and Compounds against Klebsiella pneumoniae http://www.brownlab.ca/publications-1/omdeterminants monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1583855148561-UI0K85XIETZFZ9I3MH7Q/Screen+Shot+2020-03-10+at+11.45.24+AM.png Publications - Genetic and Chemical-Genetic Interactions Map Biogenesis and Permeability Determinants of the Outer Membrane of Escherichia coli http://www.brownlab.ca/publications-1/acscannabis monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1582224148338-PEBM7TGB0AEDUPBVAYH3/Screen+Shot+2020-02-20+at+1.41.59+PM.png Publications - Uncovering the Hidden Antibiotic Potential of Cannabis http://www.brownlab.ca/publications-1/natprotpfibox monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1578956843893-6J67W7N445THMRXPO4NA/Screen+Shot+2020-01-13+at+6.06.43+PM.png Publications - A comprehensive guide to dynamic analysis of microbial gene expression using the 3D-printed PFIbox and a fluorescent reporter library http://www.brownlab.ca/publications-1/mmpxe-2021-7gjyy-sttwr-s6ylx-jwb3n-ya8xa-b7tj7-6w9kr-c274j-amzj4-g42mg-sydn5 monthly 0.5 2023-02-11 http://www.brownlab.ca/publications-1/grar monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1574782217799-S6P2772SPTRKQFS09QOU/Screen+Shot+2019-11-26+at+10.29.05+AM.png Publications - Discovery of an antivirulence compound that reverses β-lactam resistance in MRSA http://www.brownlab.ca/publications-1/om monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1574783581439-58BFQDLJUCWA5L4ZMLWM/Screen+Shot+2019-11-26+at+10.52.45+AM.png Publications - Creative targeting of the Gram‐negative outer membrane in antibiotic discovery http://www.brownlab.ca/publications-1/biotin monthly 0.5 2026-04-03 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1573772702549-GTU8BLKAI2VBKOYS4URD/Screen+Shot+2019-11-14+at+6.04.21+PM.png Publications - Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens http://www.brownlab.ca/publications-1/spr741 monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1573772899306-9LQVKYDY2ONK9BV04YS3/spr741.gif Publications - Potentiation of Antibiotics against Gram-Negative Bacteria by Polymyxin B Analogue SPR741 from Unique Perturbation of the Outer Membrane http://www.brownlab.ca/publications-1/drugrepurposing monthly 0.5 2025-07-23 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1573773382034-5OI6O8GYG49D6MBUBMHI/Screen+Shot+2019-11-14+at+6.16.09+PM.png Publications - Drug repurposing for antimicrobial discovery. http://www.brownlab.ca/publications-1/2019/01/14 monthly 0.5 2026-04-03 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1547475907530-617T1SL5OMR25M5VNSKB/Mike+paper.PNG Publications - A macrophage-based screen identifies antibacterial compounds selective for intracellular Salmonella Typhimurium http://www.brownlab.ca/publications-1/2018/8/29/open-source-high-throughput-phenomics-of-bacterial-promoter-reporter-strains monthly 0.5 2026-04-03 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1535646811566-QBCRM5ZNTQLOLSZO5D44/shawn+cell+systems.jpg Publications - Open-Source High-Throughput Phenomics of Bacterial Promoter-Reporter Strains http://www.brownlab.ca/publications-1/2018/nutrient-stress-small-molecule-screening-platform-for-escherichia-coli monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-jbkey monthly 0.5 2026-04-03 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1523917328421-ZPRSKGV9P3PZ9OJD0KMP/chemcombinationsAbstract.jpg Publications - Chemical-Chemical Combinations Map Uncharted Interactions in Escherichia coli under Nutrient Stress http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-rddtg monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-7l6jt monthly 0.5 2026-08-11 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1523982788700-O00K0OMFFKXF0P3CGMI2/Figure1+mcr1.png Publications - Overcoming mcr-1 mediated colistin resistance with colistin in combination with other antibiotics http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-g56bw monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-tlzet monthly 0.5 2026-04-22 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1523571586796-VTA7872WSIHGJ64PY4LN/bicarbabstract.jpeg Publications - Bicarbonate alters bacterial susceptibility to antibiotics by targeting the proton motive force http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-ht6m4 monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-bwwex monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response-75wrc-788j8 monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2017/03/07 monthly 0.5 2025-07-23 http://www.brownlab.ca/publications-1/2017/03/06 monthly 0.5 2026-08-11 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1523571921469-UXF4H2O7J8M0UZIY68A3/pentamidineabstract.jpg Publications - Pentamidine sensitizes Gram-negative pathogens to antibiotics and overcomes acquired colistin resistance http://www.brownlab.ca/publications-1/2016/12/15/a-cell-based-approach-to-characterize-antimicrobial-compounds-through-kinetic-dose-response monthly 0.5 2017-01-05 http://www.brownlab.ca/publications-1/2016/12/14/structure-and-mechanism-of-staphylococcus-aureus-tars-the-wall-teichoic-acid-glycosyltransferase-involved-in-methicillin-resistance monthly 0.5 2017-01-05 http://www.brownlab.ca/publications-1/2016/12/9/identification-of-two-phosphate-starvation-induced-wall-teichoic-acid-hydrolases-provides-first-insights-into-the-degradative-pathway-of-a-key-bacterial-cell-wall-component monthly 0.5 2019-01-10 http://www.brownlab.ca/publications-1/2016/11/22/the-genome-wide-interaction-network-of-nutrient-stress-genes-in-escherichia-coli monthly 0.5 2026-04-03 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1483634981912-XE2JY6P9X6F7RY2QYKTK/image-asset.jpeg Publications - The Genome-Wide Interaction Network of Nutrient Stress Genes in Escherichia coli http://www.brownlab.ca/publications-1/2017/1/5/systematic-genetic-screens-reveal-the-dynamic-global-functional-organization-of-the-bacterial-translation-machinery monthly 0.5 2018-04-11 http://www.brownlab.ca/publications-1/2016/6/23/a-comprehensive-crispr-based-functional-analysis-of-essential-genes-in-bacteria monthly 0.5 2016-08-12 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1466711490502-QGQV6AAAOTRFRVQ4XEGP/image-asset.jpeg Publications - A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria http://www.brownlab.ca/publications-1/2016/5/23/potentiation-of-aminoglycoside-activity-in-pseudomonas-aeruginosa-by-targeting-the-amgrs-envelope-stress-responsive-two-component-system monthly 0.5 2016-06-23 http://www.brownlab.ca/publications-1/2016/6/23/a-small-molecule-screening-platform-for-the-discovery-of-inhibitors-of-undecaprenyl-diphosphate-synthase monthly 0.5 2018-04-12 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1466713152641-3OZZHBSRB0CYSZQR8DFW/image-asset.gif Publications - A Small-Molecule Screening Platform for the Discovery of Inhibitors of Undecaprenyl Diphosphate Synthase http://www.brownlab.ca/publications-1/2016/5/4/strategies-for-target-identification-of-antimicrobial-natural-products monthly 0.5 2018-04-12 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467037194410-PHH793K8SE6SZ2ZGHZSX/image-asset.gif Publications - Strategies for target identification of antimicrobial natural products http://www.brownlab.ca/publications-1/2016/6/27/structural-and-kinetic-characterization-of-diazabicyclooctanes-as-dual-inhibitors-of-both-serine-lactamases-and-penicillin-binding-proteins monthly 0.5 2016-06-27 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467039103714-1RYLABILWPOU6MIQ8ZG3/image-asset.gif Publications - Structural and Kinetic Characterization of Diazabicyclooctanes as Dual Inhibitors of Both Serine-β-Lactamases and Penicillin-Binding Proteins http://www.brownlab.ca/publications-1/2016/6/27/assembly-and-clustering-of-natural-antibiotics-guides-target-identification monthly 0.5 2016-06-27 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467036381409-IS2ICR58LYTSPEZS71ME/image-asset.jpeg Publications - Assembly and clustering of natural antibiotics guides target identification. http://www.brownlab.ca/publications-1/2016/3/15/a-robust-platform-for-chemical-genomics-in-bacterial-systems monthly 0.5 2017-01-05 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467037705289-NNZDZ4IJ9N2EDS0XQZ16/image-asset.png Publications - A robust platform for chemical genomics in bacterial systems http://www.brownlab.ca/publications-1/2016/6/23/cold-stress-makes-escherichia-coli-susceptible-to-glycopeptide-antibiotics-by-altering-outer-membrane-integrity monthly 0.5 2019-01-10 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1466714081082-R1KXN0JNMNAJ17I4IPMY/image-asset.jpeg Publications - Cold Stress Makes Escherichia coli Susceptible to Glycopeptide Antibiotics by Altering Outer Membrane Integrity. http://www.brownlab.ca/publications-1/2016/1/21/antibacterial-drug-discovery-in-the-resistance-era monthly 0.5 2016-06-27 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467038484076-D89XBL4LMF2QT0E6GY2N/image-asset.png Publications - Antibacterial drug discovery in the resistance era http://www.brownlab.ca/publications-1/2015/12/31/zinc-chelation-by-a-small-molecule-adjuvant-potentiates-meropenem-activity-in-vivo-against-ndm-1-producing-klebsiella-pneumoniae monthly 0.5 2017-01-05 http://www.brownlab.ca/publications-1/2015/12/11/chemical-modulators-of-ribosome-biogenesis-as-biological-probes monthly 0.5 2018-04-12 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467040142868-FQAB92TK4F80AZLKTF83/image-asset.png Publications - Chemical modulators of ribosome biogenesis as biological probes   Simplified overview of ribosome biogenesis. (a) In yeast, ribosome biogenesis initiates with transcription of 18S, 5.8S and 25S rRNA by RNA polymerase I (Pol I). 5S rRNA, transcribed by RNA polymerase III, is omitted for clarity. Co-transcriptional assembly and modification of the pre-43S particle occurs until the emergence of the A2 RNA cleavage site, at which time the pre-43S particle is released and transcription continues through the 60S subunit rRNA genes. The 40S and 60S subunits are then independently assembled with the assistance of ~200 assembly factors. Upon export of the pre-ribosomal particles into the cytoplasm, mature 60S subunits bind pre-40S particles in an eIF5B-dependent pre-translation quality control checkpoint before final 40S maturation. Representative ribosome assembly factors are depicted with pink, yellow and purple circles. (b) Bacterial ribosome biogenesis begins with transcription of 16S, 23S and 5S rRNA as a single transcript that is then cleaved by a series of RNases to release pre-30S and pre-50S particles. As in yeast, rRNA transcripts are co-transcriptionally assembled and modified (omitted for clarity). 30S and 50S subunits are subsequently modified by ~60 biogenesis factors and mature through multiple parallel assembly pathways before translation initiation. Unlike eukaryotic ribosome biogenesis, this process in bacteria does not necessitate a nuclear export event, and a pre-translation quality control checkpoint has yet to be observed. Representative ribosome assembly factors are depicted with pink and yellow circles. http://www.brownlab.ca/publications-1/2017/1/5/gram-negative-resistance monthly 0.5 2017-01-05 http://www.brownlab.ca/publications-1/2015/10/27/new-chemical-tools-to-probe-cell-wall-biosynthesis-in-bacteria monthly 0.5 2016-08-15 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467042007606-70W2KSVDMEPO0ETCHCFJ/image-asset.png Publications - New chemical tools to probe cell wall biosynthesis in bacteria http://www.brownlab.ca/publications-1/2015/10/27/editorial-overview-microbial-systems-biology monthly 0.5 2016-06-27 https://images.squarespace-cdn.com/content/v1/575196aeb654f9980cddf500/1467041376255-EG64DEDMW21WNOVG9GYC/image-asset.jpeg Publications - Editorial overview: Microbial systems biology. http://www.brownlab.ca/publications-1/2015/9/13/unconventional-screening-approaches-for-antibiotic-discovery monthly 0.5 2016-08-15 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